Studies on Induced Mutation Frequency in Catharanthus Roseus (L.)G. Don By Gamma Rays and EMS Individually and in Combination
M. Venkateswarlu, B. N. Susheelamma, P. Sreerama Kumar, Karampuri Subhash
Abstract
M. Venkateswarlu, B. N. Susheelamma, P. Sreerama Kumar, Karampuri Subhash
Abstract
Seeds of pink flowered (PF) and white flowered (WF) Catharanthus roseus were soaked in distilled water for 24 h and treated with gamma rays and 0.1% EMS separately and in combination. Six types of chlorophyll mutations, viz., xantha, albina, chlorina, viridis, maculata and tigrina were recovered to M2 generation of both forms. The frequency of chlorophyll mutations was found to be dependent on the dose, of gamma rays and duration of treatment with EMS. Higher frequency of chlorophyll mutations was noticed in PF, which is mutagenically more sensitive than WF. It was also noticed that the combination treatments of gamma rays and EMS enhanced the frequency of chlorophyll mutations.
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Seeds of pink flowered (PF) and white flowered (WF) Catharanthus roseus were soaked in distilled water for 24 h and treated with gamma rays and 0.1% EMS separately and in combination. Six types of chlorophyll mutations, viz., xantha, albina, chlorina, viridis, maculata and tigrina were recovered to M2 generation of both forms. The frequency of chlorophyll mutations was found to be dependent on the dose, of gamma rays and duration of treatment with EMS. Higher frequency of chlorophyll mutations was noticed in PF, which is mutagenically more sensitive than WF. It was also noticed that the combination treatments of gamma rays and EMS enhanced the frequency of chlorophyll mutations.
Key concepts: Catharanthus roseus, Mutation frequency, Chlorophyll, Biology, Distilled water, Botany, Mutation breeding, Horticulture