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2011•Chinese Journal of Health Laboratory TechnologyRequires access

Effect of vitamin D receptor(VDR) FokI polymorphism on VDR protein levels in females

Youze Huang, Yongheng Bai, Lu Hong, Yunxiang Zeng, Yile Chen, Yifei Pan

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Abstract

Objective:To explore the effect of FokI polymorphisms of the VDR gene on VDR protein levels in females.Methods: A total of 229 females were enrolled in our study,and the genotypes were detected by Polymerase chain reaction-restrained fragment length polymorphism technique(PCR-RFLP).The levels of VDR protein were measured by enzyme-linked immunosorbent assay(ELISA).Results: The frequency of FokI genotype followed the Hardy-Weinberg equilibrium test.The distribution of FokI Ff genotype is most,and the frequency was 50.26%.Plasma VDR levels in females with FF genotype was 82.67±34.14 pmol/ml,significantly lower than that in females with Ff(103.78±17.48 pmol/ml) or ff genotype(114.73±53.00 pmol/ml).Additionally,the VDR levels also showed an association with age.Conclusion: The FokI polymorphism of VDR gene was associated with plasma VDR levels in females,and those with FF genotype showed lower plasma VDR levels.

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What this paper is about

Objective:To explore the effect of FokI polymorphisms of the VDR gene on VDR protein levels in females.Methods: A total of 229 females were enrolled in our study,and the genotypes were detected by Polymerase chain reaction-restrained fragment length polymorphism technique(PCR-RFLP).The levels of VDR protein were measured by enzyme-linked immunosorbent assay(ELISA).Results: The frequency of FokI genotype followed the Hardy-Weinberg equilibrium test.The distribution of FokI Ff genotype is most,and the frequency was 50.26%.Plasma VDR levels in females with FF genotype was 82.67±34.14 pmol/ml,significantly lower than that in females with Ff(103.78±17.48 pmol/ml) or ff genotype(114.73±53.00 pmol/ml).Additionally,the VDR levels also showed an association with age.Conclusion: The FokI polymorphism of VDR gene was associated with plasma VDR levels in females,and those with FF genotype showed lower plasma VDR levels.

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Available abstract

Objective:To explore the effect of FokI polymorphisms of the VDR gene on VDR protein levels in females.Methods: A total of 229 females were enrolled in our study,and the genotypes were detected by Polymerase chain reaction-restrained fragment length polymorphism technique(PCR-RFLP).The levels of VDR protein were measured by enzyme-linked immunosorbent assay(ELISA).Results: The frequency of FokI genotype followed the Hardy-Weinberg equilibrium test.The distribution of FokI Ff genotype is most,and the frequency was 50.26%.Plasma VDR levels in females with FF genotype was 82.67±34.14 pmol/ml,significantly lower than that in females with Ff(103.78±17.48 pmol/ml) or ff genotype(114.73±53.00 pmol/ml).Additionally,the VDR levels also showed an association with age.Conclusion: The FokI polymorphism of VDR gene was associated with plasma VDR levels in females,and those with FF genotype showed lower plasma VDR levels.

Key concepts: FokI, Calcitriol receptor, Genotype, Internal medicine, Endocrinology, Polymorphism (computer science), Restriction fragment length polymorphism, Vitamin D and neurology

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