Standardization of micro-propagation technique for acid lime (Citrus aurantifolia Swingle)

In an endeavour to standardize the protocol for micro-propagation of acid lime maximum survival of explants was found when the explants were grown on media comprising of BAP (0.5 mg l−1and 1.0 mg l−1), Kinetin (0.5 mg l−1), BAP (0.5 mg l−1) + NAA (0.5 mg l−. Minimum time required to bud break (27.45 days), maximum shoot (8.40) and maximum length of shoot (2.65 cm) were recorded BAP (0.5 mg l−1) + NAA (0.5 mg l−1). As regard to rooting parameters, the maximum (100 per cent) survival of micro shoot for rooting, minimum time required to sprout the bud (18.50 days) and maximum roots (4.80) were recorded in medium supplemented with IBA (0.5 mg l−1). Maximum length of root (5.80) was observed on MS medium supplemented with NAA (0.5 mgl−1) + IBA (0.5 mgl−1). The In-vitro propagated plantlets got acclimatized successfully by transferring them in small pots containing a potting mixture of soil: perlite: vermiculite in equal proportion. Following it 90 per cent survival of plantlets was observed.