[Detection and clinical significance of vascular endothelium growth factor and microvessel density in patients with nasopharyngeal carcinoma].

OBJECTIVE: To investigate the role of VEGF in angiogenesis of nasopharyngeal carcinoma tissue. METHOD: Serum and tissue VEGF were detected by the quantitative enzyme-linked immunosorbent assay (ELISA) method both in 62 nasopharyngeal carcinoma without therapy and 20 nasopharyngitis. The expression of microvessel density in tissue of nasopharyngeal carcinoma and nasopharyngitis were detected by immunohistochemical method. RESULT: (1) There was no significant relationship compared VEGF and MVD in nasopharyngeal carcinoma patient, gender and ages (P>0.05), while the expression of VEGF and MVD in later stage (III+IV) were significant higher than that in earlier stage (I+I) (P<0.01), and in positive lymph node group or metastasis group were higher than in negative group (P<0.01); (2) The expression of VEGF and MVD in nasopharyngeal carcinoma serum and tissue were higher than in nasopharyngitis (P<0.05); (3) There was positive correlation between VEGF and MVD in nasopharyngeal carcinoma tissue (r=0.865, P<0.01), but negative correlation in their serum (r=0.328, P>0.05). CONCLUSION: VEGF could induce angiogenesis in nasopharyngeal carcinoma tissue, and play an important role in progression in nasopharyngeal carcinoma, VEGF could be an important marker for monitoring prognosis of disease.