Eileen L. Whiteman, Morris J. Birnbaum
Abstract
This chapter describes techniques to successfully detect tyrosine phosphorylation of the insulin receptor and insulin receptor substrate proteins. These assays demonstrate whether the insulin signaling pathway is activated at its earliest stages.
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This chapter describes techniques to successfully detect tyrosine phosphorylation of the insulin receptor and insulin receptor substrate proteins. These assays demonstrate whether the insulin signaling pathway is activated at its earliest stages.
Key concepts: Insulin receptor, Insulin receptor substrate, IRS2, Tyrosine phosphorylation, Insulin, Phosphorylation, Receptor tyrosine kinase, GRB10