Expression difference of starch granule proteins in wheat and its relationship with starch paste characteristics.

To study the relationship between starch granule proteins and starch paste characteristic in wheat,18 leading wheat cultivar in Henan province were tested.The results indicated that 8 major starch granule proteins,9 types of patterns were presented in the 18 wheat varieties.The type 1 and type 7 with 7 bands of starch granule proteins accounted for 55.56% of the total wheat varieties.The correlation analysis between the density of bands of starch granule proteins and starch paste characteristic showed that the total band density was negatively and significantly correlated with amylase content,peak viscosity,through viscosity and final viscosity.The expression of starch granule proteins and starch paste characteristics of starch extracted with salt,SDS and protean solvent were studied in 6 wheat varieties.Compared with control,starch peak viscosity increased in starch extracted with different solvent and the highest increasing rate was presented in salt treatment.The SDS-PAGE analysis showed that the bands of starch granule proteins decreased significantly after starch was treated with different solvent.It showed that the expression of starch granule proteins in different wheat varieties was different and the amount of expression affects starch peak viscosity.