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Manual for Starch Gel Electrophoresis: A Method for the Detection of Genetic Variation

Paul B. Aebersold, Gary A. Winans, David J. Teel, G. B. Milner, Fred M. Utter

Abstract

The procedure to conduct horizontal starch gel electrophoresis on enzymes is described in detail. Areas covered are (I) collection and storage of specimens, (2)preparation of tissues, (3) preparation of a starch gel, (4) application of enzyme extracts to a gel, (5) setting up a gel for electrophoresis, (6) slicing a gel, and (7)staining a gel. Recipes are also included for 47 enzyme stains and 3 selected gel buffers. (PDF file contains 26 pages.)

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Key concepts: Gel electrophoresis, Two-dimensional gel electrophoresis, Chromatography, Polyacrylamide gel electrophoresis, Starch, Gel electrophoresis of proteins, Slicing, Electrophoresis