2007•Anhui nongye kexueRequires access
Study on Embryogenic Callus Induction and Plant Regeneration from Mature Embryo of Wheat
Jie He, Chang Jingling
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Abstract
Embryogenic callus induction and plant regeneration was conducted for the first time from mature embryos of wheat in this study.The result showed that the optimal medium for embryogenic callus induction was MS +2,4-D(3.0 mol/L) + VB1(1.0 mg/L),and the frequency of induction was 72.3 %.But the quality of embryogenic callus was poor.By reducing consistence of 2,4-D and adding ABA in subculture medium,the quality of embryogenic callus was improved.The medium for differentiation was MS + 6-BA(1.0mg/L) + KT(4.0 mg/L) + NAA(0.3 mg/L),and the differentiation frequency of callus was 73.18 %.Medium for regenerated plantlet rooting was 1/2 MS medium free of hormone.An effective system of plant regeneration of wheat was established.