Classification and Analysis of 30 Camellia Germplasm Resources by Using RAPD Molecular Markers
Zuo Zuozuo, Huang Hualin, Jiaxian Li, HE Yumei
Abstract
Zuo Zuozuo, Huang Hualin, Jiaxian Li, HE Yumei
Abstract
[目的]对30份山茶属种质资源进行分类分析.[方法]利用RAPD分子标记技术研究30份山茶属种质资源的遗传多样性,并进行分类分析.[结果]17个RAPD引物共扩增出138条带,多态性条带为129条,多态性比率为93.5%,材料间的遗传相似系数(GS)变化范围为0.605 ~ 0.855.[结论]利用NTSYS软件对RAPD扩增结果进行聚类分析,可将30份资源分为5大类群,其分类结果与张宏达分类体系基本一致.
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[目的]对30份山茶属种质资源进行分类分析.[方法]利用RAPD分子标记技术研究30份山茶属种质资源的遗传多样性,并进行分类分析.[结果]17个RAPD引物共扩增出138条带,多态性条带为129条,多态性比率为93.5%,材料间的遗传相似系数(GS)变化范围为0.605 ~ 0.855.[结论]利用NTSYS软件对RAPD扩增结果进行聚类分析,可将30份资源分为5大类群,其分类结果与张宏达分类体系基本一致.
Key concepts: Germplasm, RAPD, Camellia, Biology, Computational biology, Biotechnology, Genetics, Botany