번식생리 및 내분비 ; 돼지난포란의 난구세포 팽화는 과립막세포에 의해 억제된다

We tested the current popular hypothesis that sulfated glycosaminoglycans (GAGS) in murine follicular fluid may inhibit the precocious expansion of the cumulus oophorus in the antral follicle before the LH surge. Using porcine follicular oocytes, the effect of GAGS as a form of follicular fluid was examined in the cumulus exapansion. Degree of expansion of the cumulus-oocyte complexes cultured in porcine follicular fluid was greater then that in medium supplemented with 10% fBS and 0.02 Au/㎖ FSH. However, the cumulus oophorus of native oocytes tightly connected with mural granulosa cells in hemi-sec ted follicles remained at intact cumulus oophorus after incubation in the above conditions. In contrast to the results in mice, heparin could not inhibit cumulus expansion in pig, despite of higher concentrations (range: 100㎍∼10㎎/㎖) than those of total GAGS in porcine follicular fluid. The results indicate that GAGS may not be a main factor in regulation of cumulus oophotus during follicular development in pig, and the maintenance of tight cumulus oophorus may be due to a factors) from mural granulosa cells and contact between membrana granulosa and cumulus cells.