創傷性休克兔血漿一氧化氮、TNF-α、IL-6和血流動力學的動態變化及亞甲藍的干預作用

Objective To observe the changes of plasma nitric oxide, tumor necrosis factor alpha (TNF-α), interleukin-6 (IL-6) and hemodynamics in rabbits with traumatic shock and the intervention of methylene blue (MB). Methods Eighteen rabbits was randomly assigned into 3 groups (n=6), namely control group, traumatic shock resuscitation with normal saline group (NS group) and traumatic shock resuscitation with methylene blue group (MB group). The systolic blood pressure (SBP), diastolic blood pressure (DBP) , mean arterial pressure (MAP) and heart rate (HR) were recorded, and NO, TNF-α and IL-6 of plasma were measured, at pre-shock, tele-shock,tele-resuscitation,0.5 hour, 2 hours and 4 hours after resuscitation. Results The levels of plasma NO, TNF-α and IL-6 in rabbits after traumatic shock were very higher than pre-shock. In NS group, the levels of plasma NO, TNF-α and IL-6 after resuscitation were progressively increased, and the blood pressure after resuscitation was unstable. But in MB group, the levels of plasma NO, TNF-α and IL-6 after resuscitation were obviously decreased, and the blood pressure after resuscitation was very stable. In control group, the levels of plasma NO, TNF-α, IL-6 and hemodynamics had no changes among all courses. Conclusions NO, TNF-α and IL-6 play important roles in the pathological process of traumatic shock, and the application of MB after resuscitation can decrease plasma NO, TNF-α and IL-6 levels and ameliorate hemodynamics. MB may improve the condition of traumatic chock.