In Vitro Rumen Fermentation as Affected by Differen Levels of Pure Triglyceride Supplementation in the Diet

An in vitro experiment was conducted to study the effect of different levels of pure triglycerides (triolein) on pH, TVFA, NH3-N, molar proportions of volatile fatty acids, IVDMD, total protozoa and bacterial population. Wheat straw and concentrate mixture (60:40) were mixed and supplemented with 0,2,4,6,8 and 10% of pure triglyceride (triolein). The peak IVDMD, NH,-N, TVFA production and bacterial & protozoal counts were observed at 6% level of tryglycerlde inclusion under in-vitro conditions.