2003•Humana Press eBooksRequires access
Direct and Indirect In Situ PCR
Klaus Hermann Wiedorn, Torsten Goldmann
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Abstract
In recent years, the development of in situ technologies has made good progress. In situ hybridization (ISH) has become an important tool and has enabled the pathologist to demonstrate infectious pathogens or mRNAs in tissue sections or cytospins without destruction of morphology, thus enabling the assignment of signals to individual cells or cell compartments (, , , , , , , , ).