THE FATE OF C$sup 14$BROMOACETIC ACID IN THE MOUSE

The distribution of radioactivity in various organs and tissues of mice was studied by injecting intravenously some sodium bromoacetate-2-C/sup 14/ with a dose corresponding to a LD/sub 50/ (31 mg/kg of body weight). The radioactivity in the blood was then followed as a function of time. Examining with paper chromatography some deproteinized blood samples, taken at different times, it was possible to observe the complete disappearance of bromoacetate from the blood within 20 minutes from the injection. Therefore the measurements concerning the fate of the drug were carried out after 30 minutes from the administration. At this time, the blood radioactivity is found chiefly in the liquid part as non-protein compounds. The part bound to the proteins seems homogeneously distributed among the different fractions; the proteins to which radioactivity is bound showed an electrophoretic mobility remarkably lower than the one showed by the normal fractions. In the extracts of muscular proteins with low ionic strength, radioactivity appears uniformly distributed among the different protein components. A table is included which shows the radioactivity values in the different organs and tissues. (auth)