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2010•Asian Journal of Research in ChemistryRequires access

Simultaneous Estimation of Nitazoxanide and Ofloxacin by RP-HPLC Method

B. Jayalakshmi, Jagannathamurthy Ramesh, M. Navin Kumar, R. Vijayamirtharaj

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Abstract

The present paper deals with the development of RP-HPLC method for the determination of Nitazoxanide and Ofloxacin in bulk and in formulation using UV detector. Selected mobile phase was a combination of 0.005M tetra butyl ammonium hydrogen sulphate and acetonitrile in the ratio of 45:55and the wavelength selected was 240nm. The flow rate 1.0ml/min, and the injection volume was 20μl. The sepration was performed at ambient temperature. Retention time of Nitazoxanide and Ofloxacin was found to be 6.051and 2.106. Linearity of the method was found to be 400 to 600 mcg/ml and 160 to 240 mcg/ml for Nitazoxanide and Ofloxacin respectively. Accuracy of the method was determined through recovery studies by adding known quantities of standard drug to the pre analyzed test solution and was found to be 99.08-99.81% and 100.18-100.65% for Nitazoxanide and Ofloxacin respectively. This method was validated according to ICH guidelines.

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What this paper is about

The present paper deals with the development of RP-HPLC method for the determination of Nitazoxanide and Ofloxacin in bulk and in formulation using UV detector. Selected mobile phase was a combination of 0.005M tetra butyl ammonium hydrogen sulphate and acetonitrile in the ratio of 45:55and the wavelength selected was 240nm. The flow rate 1.0ml/min, and the injection volume was 20μl. The sepration was performed at ambient temperature. Retention time of Nitazoxanide and Ofloxacin was found to be 6.051and 2.106. Linearity of the method was found to be 400 to 600 mcg/ml and 160 to 240 mcg/ml for Nitazoxanide and Ofloxacin respectively. Accuracy of the method was determined through recovery studies by adding known quantities of standard drug to the pre analyzed test solution and was found to be 99.08-99.81% and 100.18-100.65% for Nitazoxanide and Ofloxacin respectively. This method was validated according to ICH guidelines.

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Available abstract

The present paper deals with the development of RP-HPLC method for the determination of Nitazoxanide and Ofloxacin in bulk and in formulation using UV detector. Selected mobile phase was a combination of 0.005M tetra butyl ammonium hydrogen sulphate and acetonitrile in the ratio of 45:55and the wavelength selected was 240nm. The flow rate 1.0ml/min, and the injection volume was 20μl. The sepration was performed at ambient temperature. Retention time of Nitazoxanide and Ofloxacin was found to be 6.051and 2.106. Linearity of the method was found to be 400 to 600 mcg/ml and 160 to 240 mcg/ml for Nitazoxanide and Ofloxacin respectively. Accuracy of the method was determined through recovery studies by adding known quantities of standard drug to the pre analyzed test solution and was found to be 99.08-99.81% and 100.18-100.65% for Nitazoxanide and Ofloxacin respectively. This method was validated according to ICH guidelines.

Key concepts: Nitazoxanide, Ofloxacin, Chromatography, Chemistry, Acetonitrile, High-performance liquid chromatography, Medicine, Ciprofloxacin

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