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2013•BIOINFOLET - A Quarterly Journal of Life SciencesRequires access

Induced mutational studies in M1 generation of pigeon pea

S. M. Sangle, V. Kothekar

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Abstract

Effect of EMS and SA and gamma rays on seeds of two pigeonpea varieties Amol (BDN-708) and Vaishali (BSMR- 853) was studied. The different parameters studied in M1 generation were seed germination percentage, seedling height, chlorophyll deficient sectors, leaf morphological changes, pollen sterility and survival of plants at maturity. The different types of chlorophyll chimeras observed were yellow, light green, yellowish green and dull green. All these chimeras were found to be affecting the leaflets totally, partially and at the margins. The maximum pollen sterility (10.87%) was observed at 0.020% SA in variety BSMR 853. The survival of plants at maturity showed a gradual decrease with increasing concentration/dose of mutagenic treatments.

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What this paper is about

Effect of EMS and SA and gamma rays on seeds of two pigeonpea varieties Amol (BDN-708) and Vaishali (BSMR- 853) was studied. The different parameters studied in M1 generation were seed germination percentage, seedling height, chlorophyll deficient sectors, leaf morphological changes, pollen sterility and survival of plants at maturity. The different types of chlorophyll chimeras observed were yellow, light green, yellowish green and dull green. All these chimeras were found to be affecting the leaflets totally, partially and at the margins. The maximum pollen sterility (10.87%) was observed at 0.020% SA in variety BSMR 853. The survival of plants at maturity showed a gradual decrease with increasing concentration/dose of mutagenic treatments.

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Available abstract

Effect of EMS and SA and gamma rays on seeds of two pigeonpea varieties Amol (BDN-708) and Vaishali (BSMR- 853) was studied. The different parameters studied in M1 generation were seed germination percentage, seedling height, chlorophyll deficient sectors, leaf morphological changes, pollen sterility and survival of plants at maturity. The different types of chlorophyll chimeras observed were yellow, light green, yellowish green and dull green. All these chimeras were found to be affecting the leaflets totally, partially and at the margins. The maximum pollen sterility (10.87%) was observed at 0.020% SA in variety BSMR 853. The survival of plants at maturity showed a gradual decrease with increasing concentration/dose of mutagenic treatments.

Key concepts: Sterility, Pollen, Seedling, Biology, Germination, Chlorophyll, Botany, Horticulture

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