흰쥐에서 총담관 폐쇄후의 간내담도에 대한 투과 전자 현미경적 관찰

Alteration of bile canalicular structure are known to occur in both intra-and extrahepatic cholestasis that develop in men as well as experimental animals. In order to evaluate the biochemical and ultrastructural characteristics of cholestasis in the liver of the rats, the authors conducted liver function test and examined the fine structural changes of intrahepatic biliary tract both in norrnal state and after 4 different time periods of comrnon bile ducts ligation with the transmission electron microscope. The serum SGPT began to rise irnmediately after the cornrnon bile duct ligation, showing highest value in the 24 hours group and the value decreased thereafter. The total bilirubin, total cholesterol, , r-GTP and alkaline phosphatase began to rise rapidly after the common bile duct ligation, showing highest values in the 1 week experimental group and the values decreased thereafter. After the common bile duct ligation, changes of the liver were most prominent in the bile canaliculi which showed typical structural alterations. There was the progressive dilatation of bile canaliculi with the loss of microvilli sometimes forming sacculation and diverticulation. Prominent ultrastructural changes of bile ductules after bile duct ligation were persistent bleb formations arid decreased number of rnicrovilli of luminal surface of ductular epithelial cells. The ductular epithelium showed atrophic changes and wideninp of intercellular space with abnormal microvilli was also observed.