2015•Research Archive of Indian Institute of Technology Hyderabad (Indian Institute of Technology Hyderabad)Open access

Quantitative phase imaging of cells using White Light Diffraction Phase Microscopy

S K Kalva

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Abstract

Quantitative phase imaging (QPI) techniques are very advantageous compared to the qualitative phase imaging techniques like bright field microscopy, phase contrast microscopy, differential interference contrast microscopy as the QPI techniques render phase information quantitatively whereas qualitative phase imaging techniques yield phase information qualitatively and cannot provide exact phase values. In QPI techniques, there is no need of \nexogenous contrast agents to stain or tag the specimen. White light diffraction phase microscopy (wDPM) is one of the QPI techniques which is used to quantify the phase information from the samples.

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Quantitative phase imaging (QPI) techniques are very advantageous compared to the qualitative phase imaging techniques like bright field microscopy, phase contrast microscopy, differential interference contrast microscopy as the QPI techniques render phase information quantitatively whereas qualitative phase imaging techniques yield phase information qualitatively and cannot provide exact phase values. In QPI techniques, there is no need of \nexogenous contrast agents to stain or tag the specimen. White light diffraction phase microscopy (wDPM) is one of the QPI techniques which is used to quantify the phase information from the samples.

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Available abstract

Quantitative phase imaging (QPI) techniques are very advantageous compared to the qualitative phase imaging techniques like bright field microscopy, phase contrast microscopy, differential interference contrast microscopy as the QPI techniques render phase information quantitatively whereas qualitative phase imaging techniques yield phase information qualitatively and cannot provide exact phase values. In QPI techniques, there is no need of \nexogenous contrast agents to stain or tag the specimen. White light diffraction phase microscopy (wDPM) is one of the QPI techniques which is used to quantify the phase information from the samples.

Key concepts: Phase imaging, Differential interference contrast microscopy, Microscopy, Bright-field microscopy, Phase contrast microscopy, Phase (matter), Interference microscopy, Optical microscope

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