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2007•Indian Journal of Poultry ScienceRequires access

Cloning of fowl adenovirus-4 DNA in a plasmid vector

Monika Rai, Sunanda Singh, A. Rai

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Abstract

The DNA was isolated from fowl adenovirus-4 recovered in India and digested with restriction enzyme PstI and BamHI. The digested viral DNA was analyzed by 0.7% agarose gel electrophoresis. Digestion of FAV-4 DNA with PstI enzyme produced seven fragments of 17.38, 11.80, 4.62, 3.79, 3.27, 2.02 and 1.75 kb while digestion with BamHI gave eight fragments of 12.65, 11.22, 7.5 4.48, 3.35, 3.05, 1.92 and 1.75 kb sizes. Some bands were not visible in lower portion. The average size of FAV-4 DNA was 45 kb.The PstI and BamHI digested DNA fragments were cloned in pUC18 cloning vector.

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What this paper is about

The DNA was isolated from fowl adenovirus-4 recovered in India and digested with restriction enzyme PstI and BamHI. The digested viral DNA was analyzed by 0.7% agarose gel electrophoresis. Digestion of FAV-4 DNA with PstI enzyme produced seven fragments of 17.38, 11.80, 4.62, 3.79, 3.27, 2.02 and 1.75 kb while digestion with BamHI gave eight fragments of 12.65, 11.22, 7.5 4.48, 3.35, 3.05, 1.92 and 1.75 kb sizes. Some bands were not visible in lower portion. The average size of FAV-4 DNA was 45 kb.The PstI and BamHI digested DNA fragments were cloned in pUC18 cloning vector.

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Available abstract

The DNA was isolated from fowl adenovirus-4 recovered in India and digested with restriction enzyme PstI and BamHI. The digested viral DNA was analyzed by 0.7% agarose gel electrophoresis. Digestion of FAV-4 DNA with PstI enzyme produced seven fragments of 17.38, 11.80, 4.62, 3.79, 3.27, 2.02 and 1.75 kb while digestion with BamHI gave eight fragments of 12.65, 11.22, 7.5 4.48, 3.35, 3.05, 1.92 and 1.75 kb sizes. Some bands were not visible in lower portion. The average size of FAV-4 DNA was 45 kb.The PstI and BamHI digested DNA fragments were cloned in pUC18 cloning vector.

Key concepts: PstI, BamHI, Restriction enzyme, Cloning vector, Molecular biology, HindIII, Cloning (programming), Biology

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