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Component Analysis of Isoflavonoids in Pueraria montana and Pueraria lobata by High Performance Liquid Chromatography

Chian-Ying Yang, Chien-Chih Chen, Su-Ju Lin, Chia‐Chi Chen, Horng‐Liang Lay

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Abstract

Radix of Pueraria spp. is a popular traditional Chinese medicine. There are two major species of Pueraria in Taiwan, i.e., Pueraria montana and Pueraria lobata. The major constituents of Puerariae radix were isoflavonoids, which include puerarin, daidzin, genistin, genistein, daidzein and daidzein-4', 7- diglucoside. This study developed simultaneous analysis of multi-component HPLC method to determine the six components of isoflavonoids in root, vine, leaf, and petiole parts of P. montana and P. lobata for establishing a standard quality control of Pueraria spp. Results showed that the highest level of isoflavonoids was observed in the root and vine of P. lobata, and the daidzein-4', 7-diglucoside was only found in root and vine of P. lobata. Levels of isoflavones existed among species, as well as organs, were significantly different.

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Radix of Pueraria spp. is a popular traditional Chinese medicine. There are two major species of Pueraria in Taiwan, i.e., Pueraria montana and Pueraria lobata. The major constituents of Puerariae radix were isoflavonoids, which include puerarin, daidzin, genistin, genistein, daidzein and daidzein-4', 7- diglucoside. This study developed simultaneous analysis of multi-component HPLC method to determine the six components of isoflavonoids in root, vine, leaf, and petiole parts of P. montana and P. lobata for establishing a standard quality control of Pueraria spp. Results showed that the highest level of isoflavonoids was observed in the root and vine of P. lobata, and the daidzein-4', 7-diglucoside was only found in root and vine of P. lobata. Levels of isoflavones existed among species, as well as organs, were significantly different.

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Available abstract

Radix of Pueraria spp. is a popular traditional Chinese medicine. There are two major species of Pueraria in Taiwan, i.e., Pueraria montana and Pueraria lobata. The major constituents of Puerariae radix were isoflavonoids, which include puerarin, daidzin, genistin, genistein, daidzein and daidzein-4', 7- diglucoside. This study developed simultaneous analysis of multi-component HPLC method to determine the six components of isoflavonoids in root, vine, leaf, and petiole parts of P. montana and P. lobata for establishing a standard quality control of Pueraria spp. Results showed that the highest level of isoflavonoids was observed in the root and vine of P. lobata, and the daidzein-4', 7-diglucoside was only found in root and vine of P. lobata. Levels of isoflavones existed among species, as well as organs, were significantly different.

Key concepts: Pueraria, Daidzin, Daidzein, Lobata, Genistin, Puerarin, Kudzu, Chemistry

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