2015•Современные технологии в медицинеOpen access
Оценка уровня апоптоза наивных CD8+ T-лимфоцитов у детей с острым инфекционным мононуклеозом при активации рецепторов CD95 и DR3
Elena Nikolaevna Filatova, Oleg Vladimirovich Utkin, E. V. Anisenkova, Н. Б. Преснякова, T. D. Sycheva, Viktor V. Krasnov, Н. Е. Сенягина, Ekaterina A. Kulova, Е.И. Ефимов
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Abstract
The aim of the investigation was to estimate the relation of CD95 and DR3 receptors activation with apoptosis level of naive cytotoxic Т-lymphocytes (nCТL) in children with acute infectious mononucleosis (AIM). Materials and Methods. The test materials were peripheral blood samples of healthy children and children with AIM. nCTL were isolated by negative immunоmagnetic separation. Specific activation of CD95 and DR3 receptors was performed using monoclonal antibodies. An apoptosis level and expression of receptors were studied by flow cytometry. Results. The percentage of cell apoptosis decreased in children with AIM in freshly isolated nCTL, as well as in CD95 receptor activation compared to healthy children. nCTL apoptosis in healthy children regardless of culture conditions was accompanied by the reduced quantity of CD95+DR3– cells and CD95 expression density on their surface. In children with AIM the decrease of these indices required CD95 activation. Compared to healthy children, the percentage of CD95+DR3+ cells in children with AIM decreased in CD95 activation. In CD95 receptor activation in healthy children and children with AIM, the content of CD95+DR3+ cells correlated directly with an apoptosis level. DR3 receptor activation was accompanied neither by nCTL apoptosis level change nor the changed content of DR3+ cells in both healthy children and children with AIM. Conclusion. nCTL are less sensitive to apoptosis in children with AIM compared to healthy children. DR3 receptor activation results in no change of nCTL apoptosis level both in healthy children and children with AIM. CD95 activation in patients with AIM is accompanied by increased resistance of CD95+DR3– cells to apoptosis and the susceptibility to apoptosis of CD95+DR3+ cells. The evaluation of nCTL susceptibility to CD95-induced apoptosis in AIM can serve as a subtest to assess the state of a cell component of immune system.