Simultaneous Estimation of Amoxycillin Trihdrate and Dicloxacillin Sodium in Formulation by UV-Spectroscopy

A simple UV-Spectrophotometric method has been developed for simultaneous estimation of Amoxycillin Trihydrate and Dicloxacillin Sodium from Pharmaceutical dosage forms. Methanol: water (1:1) was used as solvent. The method involves the measurement of absorbance at two wavelengths 290 nm (λmax for Amoxycillin) and 274nm (λmax for Dicloxacillin). The linearity lies between 2–10μg/ml for Amoxycillin and 10–50 μg/ml for Dicloxacillin. The accuracy and precision of the methods were determined and validated statiscally. The method showed good reproducibility and recovered with %RSD less than 2. The method are found to be rapid, specific, precise and accurate and can be successfully applied for the routine analysis of simultaneous estimation of Amoxycilin and Dicloxacillin in bulk and combined dosage form.