Cloning and prokaryotic expression of the Schistosoma japonicum Sj22.6-LHD-Sj23 fusion gene

In this study,cDNA fragments of Sj22.6 ku and LHD-Sj23 gene of Schistosoma japonicum were amplified by specific primers,respectively,from mRNA of S.japonicum by RT-PCR,and fused together by overlap-extension PCR.The fusion gene was cloned into prokaryotic expression plasmid to construct pET28-Sj22.6-LHD-Sj23 and transformed to E.coli Rosetta DE3 cells.The recombinant protein approx 35 ku was expressed and its antigenicity was confirmed by western blot.