Study on Usage Some Kind of Agar in Making Microbiological Medium Utilize to Support Practical of Microbiology

Testing on various agar medium in order to support microbiology practical laboratory at both Biology Departement UPI & High school has been carried out. An aim of this research was to select agar medium which is effective for bacteria growth and cheap because high cost of bacto agar, has been used for practical purposed. Five agar, bacto agar as a control; agar batang; agar lembar; satelit agar and swallow agar, were tested in this experimental research. Isolated culture of Pseudomonas aeruginosa and Bacillus subtilis were inoculated on each agar medium with 3 replicate for it. A method of (metode cawan tuang) was used. The effectiveness of agar was characterized by the number of both colony. The colony was counted by using colony counter. Statistic analysis of ANAVA and DMRT (Duncan's Multiple Range Test) were used to analyse the significant difference. A result showed that all agar medium were able to support Pseudomonas aeruginosa and Bacillus subtilis growth. However, the best agar medium for growing bacteria in rank order were: bacto agar, swallow agar, satelit agar, agar batang and agar lembaran. Inclusion, powder agar (swallow) is recommended to be used for making medium agar at practical exercise of microbiology.