2004•上海第二医科大学学报：英文版Requires access
EFFECT OF TITANIUM-ALLOY PARTICLES ON EXPRESSION OF MEDIATING-RELATED GENES IN HUMAN OSTEOBLASTS
杨旭, 杨庆铭, 邓廉夫, 许福平, 张玥
Open publisher page 0 citations
Abstract
Objective To study mRNA expression of the mediating-related genes in osteoblasts after exposure to Titanium-alloy particles and cytochalasin-D. Methods Human osteoblasts were isolated from trabecular fragments and exposed to 0.01% (v/v) titanium-alloy particles for 8h. Pretreatment of osteoblasts with cytochalasin-D( 5μmol/L ) to prevent phagocytosis prior to the addition of Titanium-alloy particles, mRNA expression of RANKL, OPG, and IL-6 was semi-quantified by RT-PCR. Results Expressions of RANKL, OPG, and IL-6 mRNA at respective levels were found in osteoblasts under normal condition, which did not show significant changes in the samples treated with cytochalasin-D but without particles. Exposure of osteoblasts to particles after being treated with or without cytochalasin-D altered mRNA expression of these genes, however, an increase in the ratio of RANKL/OPG mRNA was only found in samples without cytochalasin-D treatment. Conclusion Changes of expression of RANKL, OPG, and IL-6 mRNA in osteoblasts after exposure to titanium-alloy particles were independent of phagocytosis, whereas phagocytosis has a direct effect on their ability to stimulate osteoclast differentiation and function.