球根秋海棠(Begonia × tuberhybrida Voss)不定芽誘導與植株再生之研究

The objectives of this study were to develop a tissue culture system for mass propagation of tuberous begonia (Begonia x tuberhybrida Voss). Adventitious shoots were successfully regenerated from leaf or petiole explants of hybrid tuberous begonia (Begonia x tuberhybrida Voss). Leaf and petiole explants were cultured on MS medium with NAA (0.54 or 5.4 μM) and BA (0.44, 2.22, 4.4 or 22.2 μM). Adventitious shoots were successfully regenerated and the optimum medium for adventitious shoot induction of leaf or petiole explants was MS medium supplemented with 5.4 μM NAA and 2.22 μM BA both for leaf and petiole explants, with adventitious shoot induction of 1.3 and 5.0, respectively. The adventitious shoots with original tissues were transferred to liquid MS with 3.0 g L^(-1) activated charcoal cultured for 4 weeks and then 20.3 plantlets were regenerated. After rooting and the acclimatization process, plantlets were transplanted to the greenhouse. The survival rate of regenerated plantlets was 65%. From those results, we devised an adventitious shoot regeneration system from leaf explants for rapid and efficient proliferation of tuberous begonia.