缺血预处理对大鼠缺血再灌注肾脏氧化亚氮合酶，L-选择素表达的影响

Objective: To observe the change of NO,NOS and L-selectin expression in rat renal ischemic reperfusion injury after renal ischemic preconditioning and investigate the effect of ischemic preconditioning on renal ischemic reperfusion injury，Methods: Thirty SD rats were randomly divided into three groups: sham-operated group, ischemic-reperfusion group, ischemic preconditioning group. The renal underwent60-minute ischemic and2-hour reperfusion．The level of Superoxide Dismutase (SOD), Malondial-dehyde (MDA), NO, NOS of kidney, blood in each group were examined, L-selectin expression in kidney was tested with imrnunohistochemichemistry. Results: SOD: The values in ischemic-preconditioning group were significantly higher than that in ischemic-reperfusion group (P＜0.01), MDA: The values in ischemic-preconditioning group were significantly lower than that in ischemic-reperfusion group (P＜0.01), NOS, NO: The values in ischemic-preconditioning group were significantly higher than that in ischemic-reperfusion group. The expression of L-selectin in ischemic-preconditioning group was lower than that in ischemic-reperfusion group (P＜0.05). Conclusion: Ischemic preconditioning could protect kidney from ischemic reperfusion injury, the mechanism might he that it increased NOS in kidneys and blood, so that the expression of L-selection was inhibited, with improving the SOD's activity and reducing the levels of MDA in kidney and blood.