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2010•Zhongguo yufang shouyi xuebaoRequires access

Sequence analyses of 16S rRNA genes of ten nontuberculous mycobacteria isolates from cattle.

Shumin Ge, Qian Ai-dong, Wang Tie-feng

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Abstract

In this study,the 5'-end fragments of 16S rDNA genes were amplified from 10 nontuberculous mycobacteria(NTM) isolated from cattle by PCR and sequenced.The NTM sequences were compared with the published 16S rDNA sequences and classified as follows: one for M.gilvum,one for M.fortuitum,two for M.vaccae and four for M.neoaurum 4,and the rest 2 isolates were similar to certain mycobacterial species.The importance of NTM infection in cattle should be evaluated and further study on its pathogenicity to humans and animals.

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What this paper is about

In this study,the 5'-end fragments of 16S rDNA genes were amplified from 10 nontuberculous mycobacteria(NTM) isolated from cattle by PCR and sequenced.The NTM sequences were compared with the published 16S rDNA sequences and classified as follows: one for M.gilvum,one for M.fortuitum,two for M.vaccae and four for M.neoaurum 4,and the rest 2 isolates were similar to certain mycobacterial species.The importance of NTM infection in cattle should be evaluated and further study on its pathogenicity to humans and animals.

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Available abstract

In this study,the 5'-end fragments of 16S rDNA genes were amplified from 10 nontuberculous mycobacteria(NTM) isolated from cattle by PCR and sequenced.The NTM sequences were compared with the published 16S rDNA sequences and classified as follows: one for M.gilvum,one for M.fortuitum,two for M.vaccae and four for M.neoaurum 4,and the rest 2 isolates were similar to certain mycobacterial species.The importance of NTM infection in cattle should be evaluated and further study on its pathogenicity to humans and animals.

Key concepts: Biology, 16S ribosomal RNA, Nontuberculous mycobacteria, Microbiology, Gene, Gene sequence, Sequence analysis, Pathogenicity

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Sequence analyses of 16S rRNA genes of ten nontuberculous mycobacteria isolates from cattle. — Research Paper | ScholarLens