2015•ORYZA- An International Journal on RiceRequires access

Efficacy of bioagents and botanicals against brown spot

Harish Kumar, Shafaat Ahmad, Sunil Zacharia

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Abstract

Efficacy of four bioagents and two botanicals namely Trichoderma viride, Trichoderma harzianum, Pseudomonas fluorescens, Bacillus subtilis, neem leaf extract and neem oil were evaluated against brown spot of rice under field and lab condition. In field condition bioagents and botanicals were used as seed treatment and foliar spray. The seed treatment (ST) with Trichoderma viride @ 4g/kg seed + foliar spray (FS) with Pseudomonas fluorescens @ 10 g/liter of water was found effective with less disease severity of 34.39% and higher grain yield of 68.60 q/ha. Under in vitro condition in dual culture technique, Trichoderma viride was found most effective in inhabiting the growth of Helminthosporium oryzae (61.72%). In case of botanicals, neem oil @ 3% was observed best effective in inhibiting the growth of the pathogen (54.25%) under poisoned food technique.

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What this paper is about

Efficacy of four bioagents and two botanicals namely Trichoderma viride, Trichoderma harzianum, Pseudomonas fluorescens, Bacillus subtilis, neem leaf extract and neem oil were evaluated against brown spot of rice under field and lab condition. In field condition bioagents and botanicals were used as seed treatment and foliar spray. The seed treatment (ST) with Trichoderma viride @ 4g/kg seed + foliar spray (FS) with Pseudomonas fluorescens @ 10 g/liter of water was found effective with less disease severity of 34.39% and higher grain yield of 68.60 q/ha. Under in vitro condition in dual culture technique, Trichoderma viride was found most effective in inhabiting the growth of Helminthosporium oryzae (61.72%). In case of botanicals, neem oil @ 3% was observed best effective in inhibiting the growth of the pathogen (54.25%) under poisoned food technique.

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Available abstract

Efficacy of four bioagents and two botanicals namely Trichoderma viride, Trichoderma harzianum, Pseudomonas fluorescens, Bacillus subtilis, neem leaf extract and neem oil were evaluated against brown spot of rice under field and lab condition. In field condition bioagents and botanicals were used as seed treatment and foliar spray. The seed treatment (ST) with Trichoderma viride @ 4g/kg seed + foliar spray (FS) with Pseudomonas fluorescens @ 10 g/liter of water was found effective with less disease severity of 34.39% and higher grain yield of 68.60 q/ha. Under in vitro condition in dual culture technique, Trichoderma viride was found most effective in inhabiting the growth of Helminthosporium oryzae (61.72%). In case of botanicals, neem oil @ 3% was observed best effective in inhibiting the growth of the pathogen (54.25%) under poisoned food technique.

Key concepts: Pseudomonas fluorescens, Trichoderma viride, Horticulture, Neem oil, Biological pest control, Biology, Trichoderma harzianum, Seed treatment

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