A New Method for Determination of Flubendiamide and Its Metabolite Residues in Jatropha Plant Leaves

A simple and inexpensive method was developed using solid-phase extraction, together with high performance liquid chromatographic method with UV detection for determination of flubendiamide and its metabolite (flubendiamidedesiodo) residues. The evaluated parameters include the extracts by alumina packed column using hexane: ethyl acetate solvent mixture (9:1) and acetonitrile solvent. The method was validated using leaf samples spiked with flubendiamide and its metabolite (flubendiamidedesiodo) at different fortification levels (0.03 and 0.3 μg/g). Average recoveries (using each concentration six replicates) ranged 8596%, with relative standard deviations less than 2%, calibration solutions concentration in the range 0.03-10.0 μg/mL and limit of detection (LOD) and limit of quantification (LOQ) were 0.01μg/g and 0.03μg/g respectively. Finally the leaf residue samples were re analyzed by HPLC.