2005•Unpublished venueRequires access
結核分枝桿菌耐藥基因rpoB. katG. inhA突變快速檢測方法研究
李芳芳, 方紅輝, 何林, 吳偉元, 廖生贇, 張舒林
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Abstract
Objective To develop a new membrane gene chip for rapid detection of rpoB, katG and inhA gene mutation in rifampin and/or isonicazide-resistant Mycobacterium tuberculosis clinical isolates. Method According to the sequence of H37Rv strain, we designed 16 oligonucleotide specific probes for the detection of the mutant sequences in rpoB, katG and inhA genes of Mycobacterium tuberculosis. Result Mutations were found in 50(89.3%) strains of 56 randomly selected rifampin-resistant M. tuberculosis and 24(81.0%) strains of 58 randomly selected isonicazideresistant M. tuberculosis by membrane gene chip. Conclusion Membrane gene chip technology has high sensitivity and specificity for the detection of rifampin and/or isoniazid-resistant M. tuberculosis and may be applied for clinical diagnosis.