Effect of epimedium pubescen flavonoid on expression of TNF-α and IL-1-α in bone tissue of passive smoking rats.

Objective:To explore the effect of epimedium pubescen flavonoid(EPF)on the expression of passive smoking rats.Methods:Sixty Sprague-Dawley(SD)rats(aged 2-month-old)were randomly divided into six groups(n=10),include sham control group(Group A),model group(Group B),control group(Group C),treated 1 group(Group D),treated 2 group(Group E)and treated 3 group(Group F).There were five male rats and five femle rats in each group,and the male and female rats had been fed in different cage.All the rats were given the same feedstuff.Meanwhile,Group C were given calcium 75 mg·kg-1 ·d-1 combined with VitD3 21 IU·kg-1 ·d-1 by gastrogavage for 4 months;Group D EPF 75 mg·kg-1 ·d-1 ;Group E EPF 150 mg·kg-1 ·d-1 ;Group F EPF 300 mg·kg-1 ·d-1.Except the sham control group(Group A),the other groups had been exposed daily to passive cigarette smoking for four months basing on the adytum fuming method.At the end of the 4th month,all rats were sacrificed.The expression levels of TNF-α mRNA and IL-1-α mRNA in bone tissue of the proximal tibia were tested by semi-quantitative reverse-transcription polymerase chain reaction(RT-PCR)techniques,and the cor-relation between TNF mRNA and IL-1-α mRNA were explored under the pharmacological effection of the EPF by the curve fit tech-nique.Results:The expression levels of TNF-αmRNA and IL-1-α mRNA decreased in a degree in the rats belonging to the groups with EPF.The expression levels of TNF-α had decreased markedly in Group A,Group D,Group E and Group F compared with Group B,and the difference between Group A,Group D,Group E,Group F and Group B had statistical significance(P0.05),and the difference between Group C and Group B had no statistical significance(P0.05).It had decreased in Group D,Group E and Group F compared with Group A,and the difference between Group D,Group E,Group F and Group A had no statistical significance(P0.05).There had dose-dependently decreased in Group D,Group E and Group F,and the difference among them had no statistical significance(P0.05).The difference had no statistical significance(P0.05)among the other groups.The expression levels of IL-1-α mRNA had increased remarkably in the proximal cancellous tibia of the experimental rats induced by passive smoking.The expression levels decreased in a degree in the rats belonging to the groups with EPF.It had decreased markedly in Group A,Group C,Group D,Group E and Group F compared with Group B,and the difference between Group A,Group C,Group D,Group E,Group F and Group B had statistical significance(P0.05).It had decreased in Group D,Group E and Group F compared with Group A,and the difference between Group D,Group E,Group F and Group A had no statistical significance(P0.05).There had dose-dependently decreased in Group D,Group E and Group F,and the difference among them had no statistical significance(P0.05).The difference had no statistical significance(P0.05)among the other groups.Under the pharmacological effects of the EPF through the curve fit technique,the correlation between TNF mRNA and IL-1-α mRNA were Y=0.82115+0.09343X-0.01781X2.Conclusion:The EPF can restrain in a degree the expression of TNF-α and IL-1-α in bone tissue of passive smoking rats.