THE EXPLORATION OF RELATIONSHIP BETWEEN IMMUNOHISTOCHEMISTRY OF LN,FN,p53 AND TUMOROUS INVASION MICROECOSYSTEM IN HUMAN BRAIN GLIOMA

Objective To explore the relationship between immunohistochemistry of LN,FN,p53 and TIMES in human brain glioma(BG). Methods Transmission electronic microscope(TEM) and immunohistochemistry were used to investigate the morphological characteristics of micrangiums in BG and the expression of LN,FN,p53 in BG and intracranial metastatic tumors. Results 1.It was found that base laminas beneath endothelial cell with locally or extensively thickened were intact and continuous in BG.The increasing thickness of BM was consistent with the staining of LN and FN and related to p53 immunostaining.BM of p53-protein positive cases grew thicker than that of p53-protein negative ones.2.Micrangiums BM in all BG were positive for LN and FN.The more malignant the BG was,the stronger the LN and FN staining became and the thicker the blood vessel walls grew(P0.01,P0.05,respectively).Cytoplasms instead of nuclei of endothelial cells were also positive for LN and FN and there was no staining in glioma cells.There was no staining in BM or endothelial cells in intracranial metastatic tumors except cellular membranes of scattered glioma cells.Otherwise,the staining of FN in intracranial metastatic tumors was similar to that of FN in BG.3.21/45 cases showed positive for p53-protein.The positive staining of p53 was significantly correlated with the results of LN and FN immunostaining(P0.01).The difference of p53 immunostaining between intracranial metastatic tumors and high malignant glioma was not statistically significant(P0.05).Conclusion One of the reasons that BMs in TIMES in BG thicken may be the over expression of LN and FN of brain micrangium endothelial cells.Also,the influence of p53 on TIMES is associated with the functional state of endothelial cells.Micrangiums endothelial cells may be play a role in regulating TIMES.