Microcarrier를 이용한 사람 폐세포에서 수두 바이러스의 증식

A human embryonic lung fibroblast(LuMA) cell line was established for production of a live attenuated varicella-zoster virus vaccine. Using this cell line, we have investigated the possibili ty of using microcarrier culture system for producing cell-associated and cell-free varicellazoster virus. We have also investigated the utilization of glucose and amino acids during cell culture and virus propagation in microcarrier culture. It was found that no infectious cell-free viruses appear in the culture medium, and substantial decreases in cell density was observed a cell culture time increase. The cell-free varicella-zoster virus yield in microcarrier culture was much lower than that of the stationary T-flask culture.