Correlation analysis of lipid peroxidation metabolism during florescence and flower senescence of peony

【Objective】 The paper studied the changes of active oxygen species(AOS) and related enzymes activities in petal during different stages of two peony tree cultivars.【Method】 The O-2 production rate,H2O2 content,superoxide dismutase(SOD),peroxidase(POD),ascorbate peroxidase(AsA-POD),catalase(CAT) and polyphenol oxidase(PPO) and MDA content of petal of two tree peony cultivars'Luoyanghong'and 'Huhong 'during florescence and flower senescence were detected by the methods of conuentional biochemistry.The relationships among MDA content and others index were analyzed by the simple correlation coefficient,stepwise regression and the path-coefficient.【Result】 The results showed that a rapid accmulation of MDA content induced continue to increase petal cell membrance permeability during florescence and flower senescence,there were some differences of lipid peroxidation metabolisam in 'Luoyanghong'and 'Huhong'.Significantly positive correlation existed between O-2 production and H2O2 and MDA content in 'Luoyanghong',so did between O-2 production and MDA content in 'Huhong'.Also,O-2 production had positivl amost direct effect on MDA content,but other main factors as H2O2,AsA-POD,CAT and PPO had different effect on two peony trees.【Conclusion】 The active oxygen turned into imbalance resulting in lipid peroxidation during florescence and flower senescence of two peony trees.It implied that O-2 production was the key factor affecting the membrane lipid peroxidation metabolism in two peony trees during florescence and flower senescence.