2013•International Journal of BioassaysRequires access

Antiserum to buffalo pituitary luteinizing hormone enables measurement of placental gonadotropins.

Vasudha Vasudha, Nikki Kumari, K. Muralidhar

Abstract

Rabbit antiserum to buffalo pituitary Luteinizing Hormone (buLH) was raised and characterized with respect to titer, specificity and cross reaction with potential antigens with possible shared epitopes. There was very little cross reaction exhibited by antiserum to hLH with buLH. The antiserum to buLH could cross react with equine LH and Pregnant Mare serum Gonadotropin (PMSG) but to different degrees. A competitive ELISA for the measurement of PMSG could be standardized. Similarly antiserum to buLH cross reacted with hCG also in a direct binding ELISA.

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Key concepts: Antiserum, Luteinizing hormone, Titer, Epitope, Cross reactions, Internal medicine, Endocrinology, Antigen