ScholarLens Back to search
2005•Zhongguo shouyi ke-jiRequires access

Synthesis and characterization of ractopamine artificial antigen

YU Hong-Xia, Shuming Yang

Open publisher page 2 citations

Abstract

Ractopamine HCl was activated by glutarate anhydride and was converted into ractopamine-hemiglutarate.Ractopamine-hemiglutarate was linked to carrier protein keyhole limpet hemocyanin(KLH) and bovine serum albumin(BSA) with the mixed anhydride method respectively.The KLH conjugate was used as an immunogen and the BSA conjugate was used as a coating antigen for the development of an ELISA technique.UV scanning spectrum and NMR spectrum proved that the hapten ractopamine was coupled successfully to the carrier protein.The conjugation ratio of ractopamine to KLH was 24∶1 and ractopamine to BSA was 2.5∶1 by UV spectro photometry.The KLH conjugate was used to immunize New Zealand rabbits and polyclonal antibodies against ractopamine were obtained. Titers of the antibodies were over 1∶6000 by the indirect ELISA and IC_(50)was 10ng/mL by the competitive indirect ELISA.Results showed that the artificially-synthesized antigen had immunogenicity.

About this research paper

What this paper is about

Ractopamine HCl was activated by glutarate anhydride and was converted into ractopamine-hemiglutarate.Ractopamine-hemiglutarate was linked to carrier protein keyhole limpet hemocyanin(KLH) and bovine serum albumin(BSA) with the mixed anhydride method respectively.The KLH conjugate was used as an immunogen and the BSA conjugate was used as a coating antigen for the development of an ELISA technique.UV scanning spectrum and NMR spectrum proved that the hapten ractopamine was coupled successfully to the carrier protein.The conjugation ratio of ractopamine to KLH was 24∶1 and ractopamine to BSA was 2.5∶1 by UV spectro photometry.The KLH conjugate was used to immunize New Zealand rabbits and polyclonal antibodies against ractopamine were obtained. Titers of the antibodies were over 1∶6000 by the indirect ELISA and IC_(50)was 10ng/mL by the competitive indirect ELISA.Results showed that the artificially-synthesized antigen had immunogenicity.

Why it matters

OpenAlex reports 2 citations for this work. Citation counts describe recorded attention and do not establish research quality.

Key contribution

A contribution statement is not available in the OpenAlex record.

Method / approach

Method details are not available in the OpenAlex metadata.

Main findings

Findings are not separately available in the OpenAlex metadata.

Limitations

Limitations are not available in the OpenAlex metadata.

Applications

Application details are not available in the OpenAlex metadata.

Available abstract

Ractopamine HCl was activated by glutarate anhydride and was converted into ractopamine-hemiglutarate.Ractopamine-hemiglutarate was linked to carrier protein keyhole limpet hemocyanin(KLH) and bovine serum albumin(BSA) with the mixed anhydride method respectively.The KLH conjugate was used as an immunogen and the BSA conjugate was used as a coating antigen for the development of an ELISA technique.UV scanning spectrum and NMR spectrum proved that the hapten ractopamine was coupled successfully to the carrier protein.The conjugation ratio of ractopamine to KLH was 24∶1 and ractopamine to BSA was 2.5∶1 by UV spectro photometry.The KLH conjugate was used to immunize New Zealand rabbits and polyclonal antibodies against ractopamine were obtained. Titers of the antibodies were over 1∶6000 by the indirect ELISA and IC_(50)was 10ng/mL by the competitive indirect ELISA.Results showed that the artificially-synthesized antigen had immunogenicity.

Key concepts: Ractopamine, Immunogen, Hapten, Conjugate, Keyhole limpet hemocyanin, Polyclonal antibodies, Chromatography, Bovine serum albumin

Related papers

Related paper 1Related paper 2Related paper 3
Back to paper searchBrowse research topicsOriginal source
Synthesis and characterization of ractopamine artificial antigen — Research Paper | ScholarLens