2009한국생물공학회 학술대회Requires access

The Effect of Various Carbon Source on the Cell Growth of Rhodobacter sphaeroides

Hyun Jeong Lee, Soo Youn Lee, Juyong Park, Jiho Min

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Abstract

Rhodobacter sphaeroides is non-sulfur purple photosynthetic bacteria exhibiting versatile growth capabilities in aerobic or anaerobic culture condition. In this study, seven organic acids were used in order to analyze the effect of cell growth according to various carbon sources. R. sphaeroides were cultured on sistrom’s minimal medium in the presence of acetate, citrate, lactate and 4 carbon-organic acids such as fumarate, malate, succinate and oxaloacetate during 60 hr, respectively. The result showed that growth rate under the culture condition with 4 carbon-organic acids was more increased than those with acetate, citrate and lactate. Moreover the growth rate of R. sphaeroides was drastically enhanced when used succinate as a carbon source. It means that R. sphaeroides is effectively able to use 4 carbon-organic acids as carbon source.

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What this paper is about

Rhodobacter sphaeroides is non-sulfur purple photosynthetic bacteria exhibiting versatile growth capabilities in aerobic or anaerobic culture condition. In this study, seven organic acids were used in order to analyze the effect of cell growth according to various carbon sources. R. sphaeroides were cultured on sistrom’s minimal medium in the presence of acetate, citrate, lactate and 4 carbon-organic acids such as fumarate, malate, succinate and oxaloacetate during 60 hr, respectively. The result showed that growth rate under the culture condition with 4 carbon-organic acids was more increased than those with acetate, citrate and lactate. Moreover the growth rate of R. sphaeroides was drastically enhanced when used succinate as a carbon source. It means that R. sphaeroides is effectively able to use 4 carbon-organic acids as carbon source.

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Available abstract

Rhodobacter sphaeroides is non-sulfur purple photosynthetic bacteria exhibiting versatile growth capabilities in aerobic or anaerobic culture condition. In this study, seven organic acids were used in order to analyze the effect of cell growth according to various carbon sources. R. sphaeroides were cultured on sistrom’s minimal medium in the presence of acetate, citrate, lactate and 4 carbon-organic acids such as fumarate, malate, succinate and oxaloacetate during 60 hr, respectively. The result showed that growth rate under the culture condition with 4 carbon-organic acids was more increased than those with acetate, citrate and lactate. Moreover the growth rate of R. sphaeroides was drastically enhanced when used succinate as a carbon source. It means that R. sphaeroides is effectively able to use 4 carbon-organic acids as carbon source.

Key concepts: Rhodobacter sphaeroides, Rhodospirillaceae, Chemistry, Carbon fibers, Rhodospirillales, Organic acid, Carbon source, Sulfur

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