Effect of quercetin blocking HSP70 expression on pre-moxibustion suppressing gastric mucosal cell apoptosis.

Objective To observe the effects of pre-moxibustion on gastric mucosal ulcer apoptosis index(AI) and cell apoptosis in acute gastric mucosal injuring rats by perfusing quercetin into the stomach to blocking HSP70 expression,to further prove up the cell protecting effect of moxibustion and its connection with HSP 70.Methods Forty healthy Wistar rats were randomly assigned to group A(binding control),group B(model),group C(moxibustion + model),group D(quercetin + moxibustion + model),and group E(quercetin + model).Quercetin was given by gavage in group D and E,and moxibustion at Zusanli and Liangmen acupoint in group C and D every day for 8 days.At the ninth day,acute gastric mucosal injuring model was made by ethanol in group B,C,D and E.AI was checked by TUNEL method,and HSP70 by western-blot method in gastric mucosa of all groups.Results Compared with group A,the value of AI was increased obviously(P0.05),and HSP70 expression of gastric mucosa was up-regulated in group B(P0.01).Compared with group B,the value of AI was reduced obviously(P0.05) and HSP70 expression of gastric mucosa was up-regulated obviously in group C(P 0.01).The value of AI was increased obviously in group D(P 0.01),but HSP70 expression of gastric mucosa has no change.Compared with group C,the values of AI was raised obviously,but HSP70 expression of gastric mucosa was down-regulated markedly in group D.Conclusion While HSP70 expression of gastric mucosa was up-regulated by treatment with pre-moxibustion,the cell apoptosis was suppressed in acute gastric mucosal injuring rats.But after HSP70 expression was blocked by quercetin,the effect of restraining the cell apoptosis with pre-moxibustion was canceled.This study certificates that pre-moxibustion has the gastric mucosal protecting efficacy,and the effect is related with up-regulation of HSP70 expression.