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Molecular Variations among Different Accessions of Jatropha Curcas L.

M. B. Tandel, M. U. Kukadia, M. R. Parmar, N. K. Patel

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Abstract

Molecular variations among different accessions 15 Jatropha curcas accessions were carried out. The RAPD analysis of accessions showed that the five decamer primers produced a total of 27 scorable bands. Out of them 18 were polymorphic and 9 were monomorphic. The percentage of polymorphism ranged from maximum 77.78 % showed by OPF-08 to a minimum of 60.00 % by OPF-3, OPF-5 and OPF-10. Lowest genetic similarity (0.333) was observed between accessions No. 9 and 1 and 9 and 3 and the highest genetic similarity (1.000) was among various accessions including 2, 4, 5, 6, 7, 10, 11, 12, 13, 14 and 15. The dendrogram of fifteen accessions reflected that at a similarity level of 70.00 %, the accessions were divided in two clusters. The accession No. 9 was found in second cluster having minimum similarity within other accessions whereas rests of accessions were laid in first cluster.

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What this paper is about

Molecular variations among different accessions 15 Jatropha curcas accessions were carried out. The RAPD analysis of accessions showed that the five decamer primers produced a total of 27 scorable bands. Out of them 18 were polymorphic and 9 were monomorphic. The percentage of polymorphism ranged from maximum 77.78 % showed by OPF-08 to a minimum of 60.00 % by OPF-3, OPF-5 and OPF-10. Lowest genetic similarity (0.333) was observed between accessions No. 9 and 1 and 9 and 3 and the highest genetic similarity (1.000) was among various accessions including 2, 4, 5, 6, 7, 10, 11, 12, 13, 14 and 15. The dendrogram of fifteen accessions reflected that at a similarity level of 70.00 %, the accessions were divided in two clusters. The accession No. 9 was found in second cluster having minimum similarity within other accessions whereas rests of accessions were laid in first cluster.

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Available abstract

Molecular variations among different accessions 15 Jatropha curcas accessions were carried out. The RAPD analysis of accessions showed that the five decamer primers produced a total of 27 scorable bands. Out of them 18 were polymorphic and 9 were monomorphic. The percentage of polymorphism ranged from maximum 77.78 % showed by OPF-08 to a minimum of 60.00 % by OPF-3, OPF-5 and OPF-10. Lowest genetic similarity (0.333) was observed between accessions No. 9 and 1 and 9 and 3 and the highest genetic similarity (1.000) was among various accessions including 2, 4, 5, 6, 7, 10, 11, 12, 13, 14 and 15. The dendrogram of fifteen accessions reflected that at a similarity level of 70.00 %, the accessions were divided in two clusters. The accession No. 9 was found in second cluster having minimum similarity within other accessions whereas rests of accessions were laid in first cluster.

Key concepts: Dendrogram, Jatropha curcas, Biology, RAPD, Genetic similarity, Similarity (geometry), Genetic relationship, Horticulture

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