ANTI-INFLAMMATORY AND CYTOTOXICITY EFFECTS OF SALVADORA PERSICA (MESWAK) EXTRACTS ON JURKAT T-CELLS

Salvadora persica (S. persica), Meswak, is an evergreen shrub to 6-7 m. It has many biological activities such as antipyretic, anti-inflammatory and antifungal activities. This study evaluated in vitro cytotoxic and anti-inflammatory effects of S. persica extracts on human oral Jurkat (T leukemia cells). Extracts from Meswak stick and leaves were tested in different concentrations for their cytotoxic and anti-inflammatory activities on human oral Jurkat T- cells. So treated cells viability with increasing concentrations of S. persica stick extract (0.008-0.2 μg/ml) and leaves extract (0.016-0.5 μg/ml) for 24, 48 or 72 hours was assessed by using the mitochondrial dependent reduction of yellow MTT (3-[4, 5-dimethylthiazol-2-yl]-2, 5-diphenyl tetrazolium bromide) to purple formazan. Also Enzyme-linked immunosorbent assay (ELISA) was performed on supernatants from treated Jurkat T-cells with phytohemagglutinin (PHA) and both extracts to quantify IL-6, IL-8 pro-inflammatory cytokines. Statistically significant differences were indicated by p