Quantification of Expression of Pattern Recognition Receptors (PRRs) in Human Peripheral Blood Mononuclear Cells (PBMC) by Real-Time Quantitative PCR

2 Abstract: Pattern recognition receptors (PRRs) are the main sensors of pathogen and danger signals in innate immunity. They are mainly expressed by macrophages and dendritic cells of different organs. Toll like receptors (TLRs) are the most studied and best characterized PPRs which are responsible for sensing pathogen associated molecular patterns (PAMP). The contribution of PRRs in inflammation induced by microbia l infection, tissue damage and cancer are a major topic in innate as well as adaptive immunity. In the present study, peripheral blood mononuclear cells (PBMC) of healthy individuals were used to investigate the expression level of TLR2, TLR4, MyD88 and CD14 transcript in these cells. Real-time quantitative PCR was applied to quantify the expression level of these genes in PBMC of human healthy individuals. According to our results, TLR2, TLR4, MyD88 and CD14 transcripts are expressed in PBMC of healthy individuals but the level of expression of TLR2 and TLR4 are significantly higher than the expression level of MyD88 and CD14 transcript. Detection of expression level of PPRs in PBMC might have a potential implication for diagnosis of inflammatory and immune mediated disorders.