DNA LANDMARKS FOR GENETIC RELATEDNESS AND DIVERSITY ASSESSMENT IN PAKISTANI WHEAT GENOTYPES USING RAPD MARKERS
Muhammad Faisal Siddiqui, Saeed Iqbal, Shazia Erum, Naheed Naz, Sajid Khan
Abstract
Muhammad Faisal Siddiqui, Saeed Iqbal, Shazia Erum, Naheed Naz, Sajid Khan
Abstract
DNA profiles from 10 Pakistani wheat genotypes were evaluated for diversity assessment based on RAPD markers. A total of 79 DNA fragments were generated by 10 RAPD primers, with an average of 7.9 bands primer-1. Of these, 64 fragments (81%) were polymorphic among 10 genotypes. Genetic diversity was evaluated via UPGMA cluster analysis by constructing dendrogram, which were used for the calculation of similarity coefficients between these
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DNA profiles from 10 Pakistani wheat genotypes were evaluated for diversity assessment based on RAPD markers. A total of 79 DNA fragments were generated by 10 RAPD primers, with an average of 7.9 bands primer-1. Of these, 64 fragments (81%) were polymorphic among 10 genotypes. Genetic diversity was evaluated via UPGMA cluster analysis by constructing dendrogram, which were used for the calculation of similarity coefficients between these
Key concepts: UPGMA, RAPD, Dendrogram, Similarity (geometry), Genetic diversity, Biology, Primer (cosmetics), Genotype