2012•Research Journal of Pharmacy and TechnologyRequires access

In vitro Anti bacterial Activity of Different Solvent extracts of the Plant Calotropis procera

J. Mastanaiah, Nadakuditi Prabhavathi, T. Srivani

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Abstract

The antibacterial activities of hexane, chloroform, methanol extracts of the plant Calotropis procera (Henna) was evaluated on bacterial strains like Escherichia coli, Klebsiella pneumonia, Lactobacillus acidophilus, Streptococcus aureus, Streptococcus mutans, Micrococcus, Streptococcus salivarius, Bacillus subtilis, Staphylococcus epidermidis, Streptococcus gordonii. The in vitro antibacterial activity of plant extracts was performed by agar cup plate/agar well diffusion method using streptomycin in dimethyl sulphoxide as a standard drug for the comparison of antibacterial activity. The results revealed that methanol extract shows lowest values of MIC when compared to other extracts. That means the highest anti bacterial activity exhibited by methanol extract. This study conclude that the methanol extract of plant Calotropis procera showed more significant activity against all tested bacterial organisms than that of the hexane and chloroform extracts.

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What this paper is about

The antibacterial activities of hexane, chloroform, methanol extracts of the plant Calotropis procera (Henna) was evaluated on bacterial strains like Escherichia coli, Klebsiella pneumonia, Lactobacillus acidophilus, Streptococcus aureus, Streptococcus mutans, Micrococcus, Streptococcus salivarius, Bacillus subtilis, Staphylococcus epidermidis, Streptococcus gordonii. The in vitro antibacterial activity of plant extracts was performed by agar cup plate/agar well diffusion method using streptomycin in dimethyl sulphoxide as a standard drug for the comparison of antibacterial activity. The results revealed that methanol extract shows lowest values of MIC when compared to other extracts. That means the highest anti bacterial activity exhibited by methanol extract. This study conclude that the methanol extract of plant Calotropis procera showed more significant activity against all tested bacterial organisms than that of the hexane and chloroform extracts.

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Available abstract

The antibacterial activities of hexane, chloroform, methanol extracts of the plant Calotropis procera (Henna) was evaluated on bacterial strains like Escherichia coli, Klebsiella pneumonia, Lactobacillus acidophilus, Streptococcus aureus, Streptococcus mutans, Micrococcus, Streptococcus salivarius, Bacillus subtilis, Staphylococcus epidermidis, Streptococcus gordonii. The in vitro antibacterial activity of plant extracts was performed by agar cup plate/agar well diffusion method using streptomycin in dimethyl sulphoxide as a standard drug for the comparison of antibacterial activity. The results revealed that methanol extract shows lowest values of MIC when compared to other extracts. That means the highest anti bacterial activity exhibited by methanol extract. This study conclude that the methanol extract of plant Calotropis procera showed more significant activity against all tested bacterial organisms than that of the hexane and chloroform extracts.

Key concepts: Calotropis procera, Streptococcus mutans, Antibacterial activity, Lactobacillus acidophilus, Microbiology, Traditional medicine, Staphylococcus epidermidis, Streptococcus pyogenes

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