The specific siRNA plasmids construction and their inhibition effect on expression of mcl-1 protein in liver cancer cell.

The mcl-l gene-specific siRNA plasmids were constructed and transfected into HepG2.The levels of mcl-1 mRNA and Mcl-1 protein expression were detected with the Rt-PCR(real time PCR) and WesternBlot methods,respectively.And the inhibitory effects of siRNA plasmid corresponding to two different sites on mcl-1 expression were compared.Rt-PCR results showed that both the two siRNA plasmids correspomding to two sites could reduce the levels of mcl-1 mRNA and the maximum inhibition efficiency was of 70.0%,which was much higher than that of a control group.Western-Blot results were showed that transfection of mcl-l gene-specific siRNA plasmids into HepG2.The expression of Mcl-1 protein obviously inhibit the expression of mcl-l protein with the maximum inhibition rate was 44.2%.It was concluded that the rational design of specific siRNA plasmid can significantly lower levels of mcl-1mRNA and inhibit the expression of Mcl-1 protein.