2012•Anhui nongye kexueRequires access
Study on process conditions of preparation of microporous potatoes starch by complex enzyme method.
Yang Sheng-dong, Li Bing, Ling Zhang, Xinxin Li, Bo Qu, Xi Wang, Lihua Wang
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Abstract
[Objective] The technology of using α-amylase and glucoamylase to prepare microporous potato starch was studied.[Method] Taking potato starch as raw materials,starch hydrolysis rate and the oil absorption as a measure of index,the influences of the reaction temperature,two enzymes proportion,the quantity of enzyme,the chroma of substrate,buffer solution pH and reaction time on microporous potato starch were investigated.[Result] The experimental results showed that the best technological conditions were reaction temperature 45 ℃,enzyme ratio(glucoamylase /α-amylase) 6,the quantity of enzyme(amount of enzyme and starch quality than) is 1.0%,the substrate quantity chroma of 0.14 g/ml,buffer solution pH 4,the reaction time 8 h.In such process condition,the oil adsorption rate of hydrolyzed potato starch was as high as 70.2%,starch hydrolytic ratio was 34.16%.[Conclusion] The study provided a basis for the development and utilization of microporous starch.