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2014•Unpublished venueRequires access

Molecular Characterization and Genetic Diversity among Clostridium perfringens Isolates

Sherein I. Abd El‐Moez, Dalia Hamza, Sohad M. Dorgham, Basem M. Ahmed, Eman A. Khairy, Riham H. Hedia

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Abstract

A B S T R A C T Clostridium perfringens is serious bacteria causing enteric diseases in domestic animals and food poisoning in human. A total of 80 C. perfringens strains were isolated from different hosts (Sheep, goat, cows, buffalos and human) suffered from diarrhea with an incidence77. 77%, 83.33%, 75%, 57.69% and 60% respectively. Multiplex PCR was used in order to type C. perfringens by using four sets of primers specific for toxin -producing genes of C. perfringens (alpha, beta, epsilon and iota). The results of Multiplex PCR indicated that the most predominant type was C. perfringens type A with an incidence 87.5%. Sequence analysis of C. perfringens type A isolates obtained from different animal species and human in order to detect the genetic diversity of the alpha toxin gene was investigated.

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What this paper is about

A B S T R A C T Clostridium perfringens is serious bacteria causing enteric diseases in domestic animals and food poisoning in human. A total of 80 C. perfringens strains were isolated from different hosts (Sheep, goat, cows, buffalos and human) suffered from diarrhea with an incidence77. 77%, 83.33%, 75%, 57.69% and 60% respectively. Multiplex PCR was used in order to type C. perfringens by using four sets of primers specific for toxin -producing genes of C. perfringens (alpha, beta, epsilon and iota). The results of Multiplex PCR indicated that the most predominant type was C. perfringens type A with an incidence 87.5%. Sequence analysis of C. perfringens type A isolates obtained from different animal species and human in order to detect the genetic diversity of the alpha toxin gene was investigated.

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Available abstract

A B S T R A C T Clostridium perfringens is serious bacteria causing enteric diseases in domestic animals and food poisoning in human. A total of 80 C. perfringens strains were isolated from different hosts (Sheep, goat, cows, buffalos and human) suffered from diarrhea with an incidence77. 77%, 83.33%, 75%, 57.69% and 60% respectively. Multiplex PCR was used in order to type C. perfringens by using four sets of primers specific for toxin -producing genes of C. perfringens (alpha, beta, epsilon and iota). The results of Multiplex PCR indicated that the most predominant type was C. perfringens type A with an incidence 87.5%. Sequence analysis of C. perfringens type A isolates obtained from different animal species and human in order to detect the genetic diversity of the alpha toxin gene was investigated.

Key concepts: Clostridium perfringens, Microbiology, Biology, Multiplex polymerase chain reaction, Toxin, Enterotoxin, Genetic diversity, Diarrhea

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