Callus induction and organogenesis in Capsicum annuum L. cv pusajwala and G4.

Callus induction and plant regeneration in chilli pepper (Capsicum annuum L.) cv pusajwala and G4 was investigated. Cotyledon and hypocotyl explants excised from 10-12 days old seedlings were cultured on Murashige & Skoog’s (MS) medium supplemented with different concentrations of growth regulators like 2, 4-D, NAA, BAP, and Zeatin. Both the explants were found suitable for induction of callus on MS medium supplemented with NAA at 2.0mg/l. Hypocotyl was found to be a better explant for the induction of maximum callus. Healthy multiple shoots were obtained from the cut ends of cotyledonary leaf segments for both the varieties on MS containing 1.0 mg/l BAP and 0.1 mg/l IAA. The maximum root induction from the regenerated shoots was achieved on half the strength MS medium supplemented with 0.2 mg/l IAA. The in vitro grown plantlets were successfully transplanted into soil where they flowered and produced fruits identical to those developed by control plants.