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2014•Unpublished venueRequires access

Research Article Development and Validation of New Spectrophotometric Method for the Estimation of Zidovudine in Bulk and Pharmaceutical Dosage Form

K. Divya, Pushpa Latha E, Rekha Rani K

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Abstract

A simple, accurate, precise, sensitive and a highly selective spectrophotometric method was developed and validated for the estimation of Zidovudine in bulk and pharmaceutical dosage form. The estimation was carried out at maximum wavelength of 281 nm. The linearity was found in the concentration range of 5-50 µg/mL. The correlation coefficient (r2) was 0.9992. The regression equation was found to be Y = 0.0435C + 0.0205 and % RSD was found to be 2%. The developed method was validated according to ICH guidelines and was found to be accurate and precise. The validation parameters are linearity, accuracy, precision, limit of detection, limit of quantitation, robustness and ruggedness. Thus the proposed method can be successfully applied for the estimation of Zidovudine in bulk and pharmaceutical dosage form.

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What this paper is about

A simple, accurate, precise, sensitive and a highly selective spectrophotometric method was developed and validated for the estimation of Zidovudine in bulk and pharmaceutical dosage form. The estimation was carried out at maximum wavelength of 281 nm. The linearity was found in the concentration range of 5-50 µg/mL. The correlation coefficient (r2) was 0.9992. The regression equation was found to be Y = 0.0435C + 0.0205 and % RSD was found to be 2%. The developed method was validated according to ICH guidelines and was found to be accurate and precise. The validation parameters are linearity, accuracy, precision, limit of detection, limit of quantitation, robustness and ruggedness. Thus the proposed method can be successfully applied for the estimation of Zidovudine in bulk and pharmaceutical dosage form.

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Available abstract

A simple, accurate, precise, sensitive and a highly selective spectrophotometric method was developed and validated for the estimation of Zidovudine in bulk and pharmaceutical dosage form. The estimation was carried out at maximum wavelength of 281 nm. The linearity was found in the concentration range of 5-50 µg/mL. The correlation coefficient (r2) was 0.9992. The regression equation was found to be Y = 0.0435C + 0.0205 and % RSD was found to be 2%. The developed method was validated according to ICH guidelines and was found to be accurate and precise. The validation parameters are linearity, accuracy, precision, limit of detection, limit of quantitation, robustness and ruggedness. Thus the proposed method can be successfully applied for the estimation of Zidovudine in bulk and pharmaceutical dosage form.

Key concepts: Dosage form, Linearity, Correlation coefficient, Linear regression, Zidovudine, Robustness (evolution), Chromatography, Detection limit

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Research Article Development and Validation of New Spectrophotometric Method for the Estimation of Zidovudine in Bulk and Pharmaceutical Dosage Form — Research Paper | ScholarLens