DEVELOPMENT AND VALIDATION OF RP-HPLC METHOD FOR THE SIMULTANEOUS ESTIMATION OF AMLODIPINE BESYLATE, LOSARTAN POTASSIUM AND HYDROCHLOROTHIAZIDE IN TABLET DOSAGE FORM

Objective: The present work aims to develop and validate a simple, specific, and accurate reverse phase high performance liquid chromatographic method for the simultaneous estimation of Amlodipine Besylate, Losartan Potassium and Hydrochlorothiazide. Method: The method was developed and validated using Inertsil C8 column (150mm x 4.6 mm, 5µ), with mobile phase consisting of acetonitrile: buffer (700: 500 v/v) with flow rate of 1.0 ml/minute (UV detection 254 nm). Results: The retention times of Amlodipine Besylate, Losartan Potassium and Hydrochlorothiazide were found to be 3.32 min, 2.57 min and 1.92 min respectively. Linearity was observed over a wide concentration range with fairly good sensitivity of 25-75 µg/ml for Amlodipine Besylate (R2 =0.9997), 249-747 µg/ml for Losartan Potassium (R2 =0.9991) and 62-188 µg/ml for Hydrochlorothiazide (R2 =1.000). The percentage recoveries of Amlodipine Besylate, Losartan Potassium and Hydrochlorothiazide were found to be in the range of 99-101 %, 98%-100 % & 98-101% respectively. Conclusion: The proposed method is rapid, accurate and can be used for the routine analysis of Amlodipine Besylate, Losartan Potassium and Hydrochlorothiazide in their formulations.